Figure 3

Expression of autosomal and X-linked reporter gene insertions in adult males. The upper row shows the mean β-galactosidase activity of transformants with autosomal (grey bars) and X-linked (open bars) insertions of each reporter gene construct. Each bar represents an independent insertion at a different genomic location. The lower row shows the relative expression of the lacZ gene as determined using qRT-PCR. For each construct, the expression of the lowest line is set to 1 and all other expression values are scaled accordingly. The order of the bars corresponds to that in the upper row. In all cases, autosomal expression was significantly greater than X-linked expression (Mann–Whitney test, P<0.001). Error bars indicate the s.d.