Figure 4
Detection of pre-miR172 in resynthesized allotetraploids. (a) Diagram of miRNA processing from pri-miRNA, pre-miRNA to miRNA in A. thaliana and A. arenosa. Green and blue colors in the stem-loop structure indicate sequence divergence between related species. Mature miRNAs are in red color. Species-specific primer pairs were designed for MIR172b and used for 5′ and 3′ RACE-PCR amplification. (b) Preferential accumulation of A. thaliana pri-miR172 in Allo733. The ratios of A. thaliana vs A. arenosa transcripts were determined by cloning and sequencing the amplified PCR products (indicated by arrows). Asterisks indicate alternatively spliced products in the 5′ and 3′ ends of AtMIR172.
