Figure 4

Phylogeny of DGCs homologous to that associated with FRE652. The consensus unrooted tree estimated by ML (maximum likelihood) methods is presented as a radial cladogram, and bootstrap values of >75% are shown at the nodes. The phylogenetic tree is based on the alignment of the GGDEFF domains (196 informative positions) of 169 sequences (see Supplementary Figure 2), including all the DGCs (20) annotated in the genome of S. meliloti strain GR4, which are highlighted in red, with an indication of their size and genomic location. The bacterial species harboring the DGCs used in the alignment are indicated at each external branch. Major clades and relevant clusters are also indicated and the corresponding bacterial families are shown. The branches corresponding to the Rhizobiaceae/Phyllobacteriaceae clade containing the most closely related orthologs and paralogs of FRE652-associated DGC are highlighted in red. The DGCs associated to FRE652 are indicated in dark blue and the possible paralog in strain GR4 pSymA in light blue. The additional four DGC homologs identified in strain GR4 in the Blast search within the 154 hits are indicated by an asterisk, whereas the DGC associated to FRE652 is indicated by an arrow.