Table 1 Sample locations, sample sizes and summary statistics for the mtDNA CR, nuclear S7 and nuclear microsatellites of Diplodus vulgaris

Basin	Locality	Mitochondrial CR				S7						Microsatellites
		N	n	h	π	N	n^a	π	H_e	H_o	F_IS	N	PA	H_e	H_o	F_IS	R_A
	Azores Is.	23	23	0.668±0.035	0.014±0.004	15	30	0.013	0.235	0.174	0.275	28	4	0.784	0.766	0.041	7.167
	Madeira Is.	30	30	0.940±0.006	0.017±0.002	19	38	0.017	0.276	0.266	0.035	34	7	0.816	0.784	0.054	8.911
Atlantic	Canaries Is.	21	19	0.983±0.006	0.021±0.003	11	22	0.016	0.264	0.209	0.218	22	12	0.843	0.809	0.064	9.939
	Mauritania	10	10	0.978±0.018	0.016±0.003	4	8	0.015	0.464	0.306	0.377	27	13	0.798	0.762	0.070	8.872
	Portugal C.^b	34	29	1.000±0.002	0.022±0.002	24	48	0.016	0.229	0.215	0.061	46	5	0.842	0.786	0.078	9.521
	Portugal S.^c	25	22	0.961±0.006	0.017±0.002	26	52	0.017	0.208	0.185	0.111	60	26	0.839	0.826	0.025	10.069
	Cadiz	20	20	0.984±0.006	0.013±0.002	4	8	0.013	0.514	0.600	−0.333	26	5	0.829	0.774	0.087	9.478
	Malta Is.	11	3	1.000±0.157	0.023±0.011	5	10	0.017	0.391	0.283	0.299	11	2	0.792	0.798	0.040	9.778
Mediterranean	Balearic Is.	14	11	1.000±0.012	0.017±0.003	9	18	0.016	0.366	0.333	0.094	14	7	0.829	0.857	0.003	9.642
	Palermo	28	19	1.000±0.004	0.018±0.002	10	20	0.015	0.295	0.314	−0.070	28	11	0.863	0.885	−0.007	10.104
	Torre Guaceto	11	8	0.964±0.029	0.020±0.005	10	20	0.013	0.289	0.208	0.290	11	0	0.804	0.859	−0.020	9.556

Abbreviations: CR, control region; F_IS, Wright’s inbreeding coefficient within populations; h, haplotype diversity (±s.e.); H_e, unbiased expected heterozygosity; H_o, observed heterozygosity; mtDNA, mitochondrial DNA; N, number of individuals screened; n, number of haplotypes; π, nucleotide diversity (±s.e.); PA, number of private alleles; R_A, allelic richness
Values that significantly deviate from the expected Hardy–Weinberg equilibrium are in bold, whereas underlined are reported the values that are no longer significant after performing a Bonferroni test.
^aNumber of alleles.
^bSesimbra, Tejo and Sado estuary.
^cAlgarve, Arade and Lagos.

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