Table 1 The 12 nuclear microsatellite loci used to study Nothofagus cunninghamii and their genetic diversity parameters

From: Genetic differentiation in spite of high gene flow in the dominant rainforest tree of southeastern Australia, Nothofagus cunninghamii

Name

Ta (°C)

Size range (bp)

Primer (pmol)

A

Miss (%)

Error (%)

Null (%)

HO

HE

FST

RST

ncutas02

55

172–220

1.25

21

5.1

2.8

0.25

0.33

0.78

0.03

0.01

ncutas08

55

188–216

0.05

8

1.6

0

−0.02

0.45

0.51

0.10

0.09

ncutas13

55

280–314

0.25

17

0.5

2.8

0

0.72

0.78

0.03

0.00

ncutas18

55

179–233

1.50

23

3.9

2.8

0

0.65

0.69

0.02

0.02

ncutas22

55

269–327

0.25

24

0.3

9

−0.02

0.73

0.8

0.06

0.03

ncutas03

60

311–361

0.50

24

0.9

1.4

0.07

0.72

0.89

0.04

0.05

ncutas04

60

312–348

0.75

17

3.9

1.4

0.01

0.66

0.74

0.05

0.01

ncutas06

60

310–396

1.50

31

1.7

1.4

0.06

0.76

0.94

0.03

0.03

ncutas12

60

202–250

0.15

21

0.2

1.4

−0.01

0.79

0.88

0.06

0.08

ncutas15

60

138–182

0.25

14

2.1

7.6

0.21

0.37

0.71

0.03

0.02

ncutas20

60

226–256

0.50

16

0.6

2.8

0.15

0.41

0.63

0.02

0.01

ncutas25

60

166–208

0.50

20

3

4.2

0.13

0.5

0.76

0.04

0.03

Overall

   

20

1.98

3.1

0.07

0.59

0.76

0.04

0.03

  1. Annealing temperatures (Ta), size range in base pairs, primer amount per PCR, number of alleles observed per locus (A), percentage of missing data for each locus (Miss), error rate across repeats (Error), estimated frequency of null alleles (Null), observed heterozygosity (HO), expected heterozygosity (HE) and genetic differentiation indices FST and RST per locus are shown.
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