Table 4 Amplicon regions with the most informative SNPs and their characteristics for Wolbachia infections (wMel, wMelPop and wAlbB) in field-caught Aedes aegypti from Australia, Indonesia, Vietnam and Brazil

From: Mitochondrial DNA variants help monitor the dynamics of Wolbachia invasion into host populations

Region

Diagnostic

SNP (position)

Location

wMel

wMelPop

wAlbB

COI

G→A

(531 bp)

Australia

COI a (87%)

COI a (87%)

ND4

ND5

T→C

(352 bp)

Indonesia

COI

COI, primer 4

ND4

ND4

G→A

(210 bp)

Vietnam

COI

COI, primer 4b

ND4

Primer 4

G→A

(295 bp)

Brazil

COI, ND5

COI, primer 4, ND5

ND4, ND5

  1. Abbreviation: SNP, single-nucleotide polymorphism.
  2. Diagnostic SNP type (for example, G→A designates G to A transition) and bp position within the amplicon
  3. aCOI marker for wMel/wMelPop infection is 87% informative in Australian samples.
  4. bPrimer pair 4 requires consideration of at least one more SNP in addition to the SNP at 295 bp to fully differentiate samples from Vietnam.
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