Figure 2
In situ hybridisation of example root-tip metaphase and meiotic pachytene chromosomes from WSMV-resistant (a–c, e, f and h) and WSMV-susceptible wheat lines (d and g). Hybridisation signal of total genomic DNA is shown in red and of repetitive DNA probes in green. Wheat chromosomes fluoresce blue with 4′,6-diamidino-2-phenylindole. Relevant chromosomes are identified. Whole alien chromosome arms are indicated with arrows and small segments or cross-hybridisation are indicated with arrow heads. Bar, 10 μm (a–f and h) and 20 μm (g). In situ hybridisation to further lines with alien fragment are shown in Supplementary Figure S3. (a) KS96HW10-1, (d) N02Y5163 and (e) N02Y5106: genomic Th. intermedium DNA (biotin, Alexa594, red) and 120 bp repeat pSc119.2 (digoxigenin, FITC, green). (b) N02Y5018: genomic Th. intermedium DNA (biotin, Alexa594, red) and 5S rDNA (digoxigenin, FITC, green). (c) N02Y5003: genomic rye DNA (biotin, Alexa594, red) and 340 bp repeat dpTa1/Afa (digoxigenin, FITC, green). (f) N02Y5149: Th. intermedium (digoxigenin, FITC shown in red) and 45S rDNA (biotin, Alex594 shown in green). (g) N02Y5156: Pachytene chromosomes: genomic rye DNA (biotin, Alexa594, red) and 5S rDNA (digoxigenin, FITC, green). (h) N02Y5057: genomic Th. intermedium DNA (biotin, Alexa594, red) and 340 bp repeat dpTa1/Afa (digoxigenin, FITC, green).
