Figure 3 | Heredity

Figure 3

From: Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance

Figure 3

Diagram of alien translocation and recombinant chromosomes. Examples of FISH signals of two probes each (green and red) on blue 4′,6-diamidino-2-phenylindole-stained chromosomes are given. (a) Wheat 4D and T4DL*4Ai#2S chromosomes showing the dpTa1/Afa, pSc119.2 and genomic DNA hybridisation patterns. The Th. intermedium arm has a terminal pSc119.2 site (see Figure 2e) and the dpTa1 site is more proximal than on 4D. (b) Wheat chromosome 1B, 1B recombinant (BPI and PBII) and T1BL*1RS showing the characteristic arrangement of 5S (light green), 45S rDNA (brown) and pSc119.2 sites (blue). Chromosome arm 1BS has one telomeric pSc119.2 site that is not present in all lines, while 1RS is slightly longer and has two pSc119.2 sites distal and proximal of the NOR region. The BPI and BPII recombinant chromosomes characterised by molecular markers cannot be distinguished cytologically. Note that the subtelomeric heterochromatin hybridises strongly with both rye and Th. intermedium genomic DNA.

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