Table 1 Pedigrees of reference lines and four populations of derived lines (from C. James Peterson, USDA-ARS) studied here with results of WSMV resistance field tests at different locations and years and summary of FISH/GISH results obtained with genomic Th. intermedium, rye and repetitive DNA probes

From: Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance

Reference/ population

Pedigree/source

Line/variety

Wheat chromosome with alien segment

Field response to WSMV a

   

4D

1B

?D

2002 b

2012, Mead, NE

      

Sidney, NE

Hays, KS

SPAD c Mean (std)

Visual Score

KS102d

KS91H184/KS89H20//TAM107

KS95H102

+

−

 

R

R

  

KS10-1d

KS91HW29//RioBlanco/KS91H184

KS96HW10-1

+

−

 

R

R

  

Milleniumd

Arapahoe/Abilene//NE86488

Millennium

−

−

 

S

S

  

Tomahawke

Agripro, WI88-083

Tomahawk

     

14.5 d (2.1)

S

Population I

CO850034//T-57/5 *TAM107/3/(KS91H184/ RioBlanco/KS91HW29//VISTA)

N02Y5018

+

BPI

 

R

R

  
  

N02Y5019

−

BPI

 

S

S

23.5 c (2.3)

MS

  

N02Y5021

−

−

 

S

S

  
  

N02Y5025

+

−

 

R

R

  
  

N02Y5003

−

BPIII

 

R

R

24.0 c (2.5)

MR

Population II

Yuma//T-57/3/LAMAR/4/4 *Yuma/5/(KS91H184/ArlinS/ KS91HW29//NE89526)

N02Y5057

+

−

 

R

R

  
  

N02Y5075

+

−

 

R

R

  
  

N02Y5078

+

−

 

R

R

  
  

N02Y5082

−

−

 

S

S

  
  

N02Y5096

−

−

 

S

S

  

Population III

Yuma//T-57 /3/CO850034/4/4 *Yuma/5/(KS91H184/ArlinS/ KS91HW29)//NE89526)

N02Y5105

−

−

 

S

MR

  
  

N02Y5106

+

−

 

R

MR

  
  

N02Y5109

−

−

+

R

R

15.4 d (1.7)

S

  

N02Y5117 (Mace)

+

−

 

R

R

28.5 b (6.9)

R

  

N02Y5121

−

−

 

S

S

  

Population IV

MO8/Redland//KS91H184/3 *RioBlanco

N02Y5149

+

BPIII

 

R

R

36.3 a (3.5)

R

  

N02Y5154

+

−

 

R

R

  
  

N02Y5156

−

BPIII

 

S

S

  
  

N02Y5163

−

BPII

 

S

S

  
  

N02Y2016

+

BPI

 

R

R

  
  1. Abbreviation: WSMV, Wheat streak mosaic virus. Recombinant chromosomes were disomic in all cases and included the T4DL*4Ai#2S Th. intermedium translocation (4D; see Figure 3a); an alien fragment on the short arm of 1B of classified into three breakpoint (BP) types by FISH and molecular markers (see Table 2 and Figures 3b and 4): BPI very small, BPII small and BPIII whole arm-alien fragment; and a small alien fragment on an unknown D-genome chromosome (?D; see Supplementary Figure S1).
  2. Other Sources: Wells et al., 1982; Divis et al., 2006; Graybosch et al., 2009 and personal communication.
  3. aR=resistant or no symptoms, MR=moderately resistant or slight symptom severity, MS=moderately susceptible or moderate symptom severity, S=susceptible or severe. In terms of plant phenotype, a score of 1=no loss of chlorophyll, and no stunting; 2=random and occasional yellow foci or streaks, 3=50% loss of chlorophyll and 4=leaves complete yellow and plants severely stunted.
  4. bFrom Divis et al., 2006. Scores are visual ratings, based on degree of streaking/chlorosis and stunting.
  5. cSoil Plant Analytical Development metre readings for leaf chlorophyll content; Higher SPAD readings indicate higher intensity of greenness of leaf tissue (no or mild symptoms), while lower SPAD readings indicate increasing severity of virus symptoms. Means followed by the same letter were not significantly different at P=0.05.
  6. dReference lines previously known for presence/absence of Th. intermedium chromatin and validated here.
  7. eTomahawk was used as reference susceptible to WSMV in the 2012 trial only; it was selected from a bulk population of crosses of adapted parents.
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