Figure 1
From: Incomplete offspring sex bias in Australian populations of the butterfly Eurema hecabe
Schematic for the 2008 experiment. Dams (circles) and sires (squares) are depicted with a diagonal slash if wild-caught, and duplicate crosses are collapsed to aid presentation. (a) Wild-caught females contributed an F1 generation of 1449 individuals in k=83 families. (b) Daughters from the 10 most female-biased F1 families were then outcrossed with wild-caught males; (c) F2 offspring (n=160 in k=20 broods) were reared to adulthood. Daughters from three female-biased families (tracing from F0 lineages ‘P5’, ‘P16’ and ‘P24’) were randomly assigned to control versus tetracycline (shaded) feeding treatments, then outcrossed with wild males; (d) F3 offspring (total n=446 in k=28 broods) were subsequently reared for the assessment of adult sexual phenotype.
