Figure 2
From: Incomplete offspring sex bias in Australian populations of the butterfly Eurema hecabe
Schematic for the 2009 experiment, presented as per Figure 1. (a) We reared the broods of 103 wild females, and identified 5 female-biased and 5 unbiased F1 families; (b) Daughters in each F1 family were randomly assigned to control, tetracycline or rifampicin feeding treatments (open, shaded and solid circles, respectively), then outcrossed with wild-caught males; (c) F2 offspring (n=2354 in k=79 broods) were reared to adulthood. Finally, we performed reciprocal crosses between biased and unbiased control F2 individuals, and (d) reared F3 offspring (n=85 in k=11 broods).
