Figure 5

Expression profiles of the grapevine VvWRKY genes in different grapevine organs, tissues and developmental stages. (a) Expression of VvWRKY genes in the V. vinifera cv ‘Corvina’ atlas (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=lfcrxesyciqgsjoandacc=GSE36128). Data were normalized based on the mean expression value of each gene in all tissues analysed. Genes were hierarchically clustered based on average Pearson’s distance metric and ‘average linkage’ method. Yellow and blue boxes indicate high and low expression levels, respectively, for each gene. (b) qRT-PCR validation of VvWRKY expression in roots, stems, leaves and shoot tips obtained from 4-week-old in vitro explants of the V. vinifera PN40024 genotype. Transcripts were normalized to the expression of the actin gene. The mean±s.d. of three biological replicates are presented. Asterisks indicate that the corresponding gene was significantly up- or downregulated in a given organ (*p≤0.05, **p≤0.01). Bud-AB, bud after burst; Bud-B, Bud burst; Bud-W, winter bud; Bud-L, latent bud; Bud-S, bud swell; Flower-F, flowering; Flower-FB, flowering begins; FS, fruit set; Inflorescence-Y, young inflorescence with single flowers separated; Inflorescence-WD, well-developed inflorescence; Leaf-FS, mature leaf; Leaf-S, senescing leaf; Leaf-Y, young leaf; MR, mid-ripening; R, ripening; PFS, post fruit set; Stem-G, green stem; Stem-W, woody stem; Tendril-FS, mature tendril; Tendril-WD, well-developed tendril; Tendril-Y, young tendril; V, véraison.