Table 2 The identification of differentially responsive proteins in tea leaves subjected to drought
Spot no. | Protein name | Accession no.a | Species | Theoretical | Experimental | Scoreb | %cov (matching peptides)c | ||
---|---|---|---|---|---|---|---|---|---|
Mr (kDa) | pI | Mr (kDa) | pI | ||||||
1 | 39 kDa EF-Hand containing protein | CAA04670 | Solanum tuberosum | 39.01 | 4.65 | 60.13 | 5.34 | 50 | 2%(1) |
2 | Ascorbate peroxidase | ACB45429 | Camellia sinensis | 27.75 | 5.50 | 38.58 | 5.58 | 235 | 37%(4) |
3 | ATP synthase CF1 alpha subunit | NP_862739 | Calycanthus floridus var. glaucus | 55.21 | 5.20 | 41.46 | 5.50 | 227 | 10%(3) |
4 | Chloroplast phosphoribulokinase | BAL03266 | Closterium peracerosum-strigosum-littorale complex | 45.77 | 6.28 | 55.02 | 5.76 | 195 | 12%(3) |
5 | Chloroplast phosphoribulokinase | BAL03266 | Closterium peracerosum-strigosum-littorale complex | 45.77 | 6.28 | 55.65 | 5.67 | 140 | 12%(3) |
6 | Chloroplast ribulose 1,5-bisphosphate carboxylase/oxygenase activase | ABW80752 | Flaveria bidentis | 48.81 | 6.10 | 42.69 | 5.20 | 354 | 17%(6) |
7 | Glutamine synthetase, chloroplastic | O22506 | Daucus carota | 48.08 | 5.63 | 47.88 | 5.97 | 195 | 14%(3) |
8 | Glutamine synthetase | BAD99525 | Camellia sinensis | 39.40 | 5.79 | 45.99 | 5.99 | 361 | 35%(5) |
9 | Glutathione transferase lambda 2 | NP_191064 | Arabidopsis thaliana | 33.15 | 6.78 | 39.26 | 6.29 | 61 | 4%(1) |
10 | Heat shock protein 90 | ACX42226 | Ipomoea nil | 90.65 | 4.92 | 52.91 | 5.19 | 133 | 5%(4) |
11 | Light-induced protein, chloroplastic | P80471 | Solanum tuberosum | 35.67 | 5.26 | 41.21 | 5.22 | 68 | 3%(1) |
12 | Lipoxygenase | ACJ54281 | Camellia sinensis | 102.59 | 6.39 | 49.36 | 5.67 | 91 | 4%(3) |
13 | Os05g0187000 | NP_001054834 | Oryza sativa Japonica Group | 29.26 | 6.45 | 37.22 | 6.18 | 76 | 5%(1) |
14 | PREDICTED: sedoheptulose-1,7-bisphosphatase, chloroplastic | XP_002263049 | Vitis vinifera | 42.90 | 5.95 | 52.37 | 5.62 | 427 | 18%(5) |
15 | Thioredoxin X | ACG44176 | Zea mays | 19.25 | 9.20 | 11.65 | 5.75 | 64 | 6%(1) |
16 | Ribulose-1,5-biphosphate carboxylase/oxygenase large subunit | ACY64810 | Richea scoparia | 52.11 | 6.08 | 16.66 | 5.29 | 185 | 12%(3) |
17 | Ribulose 1,5-bisphosphate carboxylase | CAQ58398 | Prismatomeris sp. 1 Davis 4057 | 52.12 | 6.34 | 18.53 | 6.06 | 275 | 12%(5) |
18 | Predicted protein | XP_002318440 | Populus trichocarpa | 26.38 | 8.12 | 17.76 | 5.54 | 277 | 19%(4) |
19 | Hypothetical protein VITISV_037064 | CAN69818 | Vitis vinifera | 61.39 | 5.20 | 83.44 | 5.43 | 421 | 11%(5) |
20 | Unknown | ABK92500 | Populus trichocarpa | 80.87 | 6.29 | 15.00 | 6.84 | 45 | 1%(1) |
21 | Unknown | ABK94111 | Populus trichocarpa | 16.59 | 5.14 | 12.60 | 6.45 | 48 | 12%(2) |