Table 1 Date specific proteins identified as differentially regulated during fruit development and ripening
From: Dynamic changes in the date palm fruit proteome during development and ripening
Spot no. | Identified protein | Protein probability | Accession no. | Theoretical MW (Da)/pI | Observed MW (Da)/pI | Sequence coverage | NUP | FC S1/MD | FC S1/NTR | FC S1/R |
|---|---|---|---|---|---|---|---|---|---|---|
01 | Metabolism | |||||||||
197 | Furostanol glycoside 26-O-β-glucosidase | 100.00% | 30s65509133g003 | 64132/5.34 | 61000/6.1 | 13.30% | 7 | ns | ns | −7.28 |
227 | Dolichyl-phosphate-mannose-glycolipid α-mannosyltransferase | 98.50% | 30s785981g002 | 64116/9.91 | 39000/5.6 | 1.44% | 1 | ns | 4.20 | 6.00 |
235 | Lysosomal α-subunit | 100.00% | 30s945081g001 | 202855/7.02 | 82000/5.35 | 1.44% | 3 | ns | −3.61 | 1.99 |
385 | Disproportionating enzymea | 99.70% | 30s1194551g005 | 52152/5.41 | 61000/5.0 | 3.70% | 1 | ns | ns | −1.91 |
482 | AMP-dependent | 100.00% | 30s993441g001 | 29388/5.16 | 66000/4.5 | 12.50% | 3 | −2.02 | 4.57 | 5.56 |
566 | Ornithine carbamoyltransferasea | 99.50% | 30s699641g001 | 42893/7.66 | 16000/4.3 | 3.08% | 1 | ns | −2.03 | −2.99 |
02 | Energy | |||||||||
477 | UMP6_mitochondrial flags: precursor | 100.00% | 30s1009951g003 | 16907/5.11 | 15000/5.95 | 13.60% | 2 | ns | −2.16 | −2.70 |
03 | Cell growth/division | |||||||||
366 | Growth regulator | 98.10% | 30s797691g002 | 19927/10.01 | 62000/5.6 | 6.29% | 1 | ns | ns | −3.21 |
387 | KU P80 DNA | 99.50% | 30s862811g001 | 75649/6.15 | 20000/4.9 | 1.48% | 1 | 2.75 | ns | ns |
04 | Transcription | |||||||||
302 | Gata transcription factor 25 | 99.70% | 30s1133641g001 | 80728/9.32 | 25000/5.65 | 1.37% | 1 | ns | 2.14 | −1.96 |
302 | Inducer of cbf expression 1 DNA binding transcription activator transcription factor | 99.70% | 30s953031g001 | 38914/10.25 | 25000/5.65 | 2.51% | 1 | ns | 2.14 | −1.96 |
05 | Protein synthesis | |||||||||
249 | Gag-pol polyprotein | 99.70% | 30s811431g002 | 49976/7.27 | 24000/4.5 | 2.04% | 1 | ns | 1.65 | 1.72 |
274 | Aspartyl-tRNA synthetasea | 100.00% | 30s6550950g017 | 66976/5.84 | 65000/4.75 | 19.30% | 7 | ns | ns | 3.30 |
06 | Protein destination and storage | |||||||||
129 | Proline iminopeptidasea | 100.00% | 30s775611g009 | 40654/5.64 | 31000/5.45 | 4.57% | 2 | ns | 2.22 | ns |
210 | Proteasome subunit β type 7-A | 100.00% | 30s1126271g001 | 29520/6.79 | 19000/4.4 | 4.40% | 1 | −2.20 | −4.21 | −4.79 |
300 | Nuclear transport | 99.20% | 30s1197741g004 | 16883/5.77 | 11000/5.25 | 6.80% | 1 | −2.91 | −3.50 | −1.66 |
370 | GDP dissociation inhibitora | 100.00% | 30s1179061g005 | 49697/5.83 | 47000/4.8 | 4.50% | 2 | ns | 1.74 | 2.60 |
531 | Chaperonin containing t-complex protein epsilon | 100.00% | 30s1179991g005 | 59114/5.67 | 30000/6.15 | 7.32% | 4 | ns | ns | 1.64 |
08 | Intracellular traffic | |||||||||
417 | Protein binding structural molecule | 99.90% | 30s784571g001 | 33386/5.26 | 50000/5.75 | 6.89% | 1 | ns | −1.93 | −2.51 |
11 | Disease/defence | |||||||||
303 | Heat shock protein 82 | 100.00% | 30s947641g003 | 80204/5.04 | 82000/5.9 | 10.70% | 2 | ns | −2.50 | −1.57 |
303 | Heat shock protein 82 | 100.00% | 30s722381g008 | 89708/5.18 | 82000/5.9 | 12.40% | 3 | ns | −2.50 | −1.57 |
344 | Heat shock protein 82 | 99.60% | 30s1082281g001, 30s722381g008, 30s947641g003 | 89708/4.66 | 80000/5.85 | 3.17% | 1 | ns | −2.73 | −1.57 |
352 | Heat shock protein 82 | 99.70% | 30s1082281g001, 30s722381g008, 30s947641g003 | 89708/4.66 | 54000/5.9 | 3.17% | 1 | ns | ns | −2.00 |
401 | Aldose reductase | 100.00% | 30s1006721g002 | 34679/6.01 | 19000/5.0 | 14.10% | 5 | 2.13 | ns | 1.78 |
12 | Unclear classification | |||||||||
91 | Riboflavin α- subunit | 99.00% | 30s703111g002 | 28760/9.09 | 19000/5.5 | 5.22% | 2 | 2.09 | −1.58 | −2.61 |
199 | Nucleic acid binding | 99.80% | 30s1050381g022 | 36142/4.99 | 22000/5.88 | 6.98% | 1 | ns | −2.95 | −2.56 |
477 | Nucleic acid binding | 100.00% | 30s1050381g022 | 14374/4.99 | 15000/5.95 | 10.90% | 2 | ns | −2.16 | −2.70 |
507 | JHL06P13.3-like proteinb | 99.80% | 30s1042771g010 | 50733/5.46 | 43000/5.3 | 2.22% | 1 | ns | 3.96 | 9.99 |
508 | JHL06P13.3-like proteinb | 100.00% | 30s1042771g010 | 50733/5.46 | 55000/6.26 | 4.44% | 2 | ns | 1.73 | ns |
561 | Mitochondrial glycoprotein | 99.20% | 30s796661g001 | 33383/5.55 | 26000/4.35 | 4.05% | 1 | ns | ns | −2.03 |
20 | Secondary metabolism | |||||||||
431 | Glutamate 1-semialdehyde aminotransferase | 100.00% | 30s1039641g001 | 50533/5.98 | 39000/4.8 | 5.29% | 2 | ns | 4.54 | 5.18 |
603 | Glutamate 1-semialdehyde aminotransferase | 99.60% | 30s1039641g001 | 50533/5.98 | 55000/5.33 | 2.54% | 1 | ns | 4.07 | 3.70 |
623 | Formamidase-like protein | 99.50% | 30s702741g005 | 48663/5.73 | 34000/4.5 | 2.47% | 1 | ns | −1.73 | −3.02 |
635 | Formamidase-like protein | 99.50% | 30s702741g005 | 48663/5.73 | 54000/5.1 | 2.47% | 1 | −1.66 | −3.63 | −4.27 |
