Table 2 Differentially regulated proteins in the date hypanthium showing contrasting transient patterns compared to other fruits

From: Dynamic changes in the date palm fruit proteome during development and ripening

Spot no.

Identified protein

Protein probability

Accession no.

Theoretical MW (Da)/pI

Observed MW (Da) /pI

Sequence coverage

NUP

FC S1/MD

FC S1/NTR

FC S1/R

01

Metabolism

         

91

Isoamyl acetate-hydrolyzing esterase

100.00%

30s696051g001

36883/5.86

19000/5.5

4.79%

2

2.09

−1.58

−2.61

192

Glucan endo-1,4-β-glucosidase

100.00%

30s896091g001

16069/6.54

60000/6.1

14.50%

3

−1.90

−2.96

−15.84

196

Cytochrome p450

99.70%

30s6550951g018

24174/4.39

23000/6.1

7.37%

1

ns

ns

−2.09

202

Transketolase 1

99.00%

30s656601g005

84589/5.66

95000/4.9

1.42%

1

1.86

−8.33

−8.45

312

UDP-glucose pyrophosphorylase

100.00%

30s904561g011

34207/6.1

54000/5.25

7.47%

2

ns

−2.28

−1.65

317

Sorbitol dehydrogenase

100.00%

30s830031g004

38361/5.53

45000/5.6

21.30%

7

ns

ns

−1.56

443

S-adenosylmethionine synthetase

100.00%

30s1183641g001

43368/5.68

43000/5.1

11.20%

3

ns

−2.25

−4.48

443

S-adenosylmethionine synthetase

100.00%

30s1207141g006

43344/5.76

43000/5.1

8.08%

1

ns

−2.25

−4.48

471

Aldehyde dehydrogenase

100.00%

30s693451g001

59067/6.48

55000/4.65

18.80%

11

ns

2.18

−2.17

476

S-adenosyl-l-homocysteine hydrolase

100.00%

30s1132381g003, 30s825931g001

38838/6.72

45000/6.0

13.00%

6

ns

ns

−2.83

476

Aldehyde dehydrogenase

100.00%

30s693451g001

59067/6.98

45000/6.0

12.20%

5

ns

ns

−2.83

522

Reversibly glycosylated polypeptide

100.00%

30s969461g002

41051/6.12

37000/5.9

3.59%

2

ns

−2.27

−3.20

573

Pyruvate decarboxylase

99.70%

30s919171g003

38883/8.66

60000/6.2

3.46%

1

ns

−2.33

−5.94

660

S-adenosylmethionine synthetase

99.60%

30s1183641g001, 30s1207141g006, 30s799761g004, 30s933141g001

43344/5.68

25000/6.1

3.28%

1

ns

ns

−2.64

02

Energy

         

2

Pyruvate dehydrogenase E1 component β-subunit

99.90%

30s852391g002

40148/5.46

34000/5.74

6.43%

1

ns

−3.12

−2.73

18

Enolase

100.00%

30s663761g002

47828/5.91

55000/5.15

7.64%

3

ns

−1.90

−1.50

403

Citrate synthase

99.50%

30s1040901g002

30711/6.25

55000/4.3

6.64%

1

ns

2.52

3.23

421

Malate dehydrogenase

100.00%

30s892681g001

48314/7.11

49000/6.25

6.11%

1

−1.64

−7.02

−4.54

461

Malate dehydrogenase

100.00%

30s903851g004, 30s946431g012

26083/8.76

30000/4.7

4.39%

2

ns

ns

−2.09

506

Pyruvate dehydrogenase E1 component β-subunit

100.00%

30s855801g002

22740/5.7

35000/5.31

13.00%

2

ns

−1.60

−1.70

531

Bisphosphoglycerate-independent phosphoglycerate mutase

100.00%

30s661041g002, 30s693071g003

43543/5.55

30000/6.15

5.60%

1

ns

ns

1.64

566

Fructose-bisphosphate aldolase

100.00%

30s1148281g010

40222/7.55

16000/4.4

12.10%

4

ns

−2.03

−2.99

577

Transaldolase-like protein

100.00%

30s1065691g004

43557/5.05

55000/6.5

4.88%

2

−1.79

−7.48

−4.49

03

Cell growth/division

         

45

Condensin complex subunit 1

99.30%

30s702551g004

127265/5.78

51000/5.34

1.47%

1

ns

2.53

1.95

252

Enhancer of polycomb-like protein

99.70%

30s827291g002

51710/9.99

18000/6.2

5.79%

1

4.51

4.99

2.11

415

14-3-3-like protein

99.70%

30s819041g002

17032/5.09

19000/6.24

7.95%

1

2.71

2.55

1.72

04

Transcription

         

416

Transcription factor IIA small subunit

99.70%

30s656261g002, 30s760301g006

12093/6.08

27000/4.7

10.40%

1

2.52

3.51

2.46

05

Protein synthesis

         

45

Translation initiation factor (eif-4a)

99.90%

30s724051g001, 30s997411g005

47064/5.39

51000/5.34

2.42%

1

ns

2.53

1.95

53

Elongation factor 1

99.20%

30s667161g002

16163/4.26

21000/6.6

11.90%

1

−1.84

−1.79

1.55

287

30s ribosomal protein s1

97.70%

30s783011g009

45242/4.95

35000/6.25

2.15%

1

1.58

8.73

11.49

416

Peptide chain release factor, putative

99.70%

30s682511g003

9163/4.85

27000/4.7

13.30%

1

2.52

3.51

2.46

504

60s ribosomal protein l23a

99.70%

30s1035671g001

9453/11.22

88000/6.0

11.00%

1

1.62

ns

−3.63

525

Translation initiation factor (eif-4a)

100.00%

30s724051g001, 30s997411g005

47064/5.39

39000/5.34

9.93%

4

ns

3.98

5.12

06

Protein destination and storage

         

3

Cysteine protease

98.80%

30s790241g001

36042/4.87

22000/6.55

4.98%

1

ns

−2.48

−1.80

32

Luminal binding protein

100.00%

30s685511g001

56109/8.42

82000/5.95

7.24%

2

ns

ns

−2.52

92

Mitochondrial processing peptidase

100.00%

30s927641g002

59451/5.76

55000/5.48

6.08%

3

ns

1.92

1.88

239

Luminal binding protein

100.00%

30s685511g001

56109/8.42

70000/5.8

18.00%

8

ns

ns

−1.62

288

Cysteine protease

99.70%

30s790241g001

36042/4.87

32000/6.3

4.98%

1

ns

2.43

2.24

303

Subtilisin-like serine proteinase

100.00%

30s808251g002

48672/4.79

82000/5.9

5.76%

2

ns

−2.50

−1.57

419

Mitochondrial processing peptidase

100.00%

30s927641g002

59451/5.76

55000/5.5

4.60%

1

ns

2.78

ns

609

Cysteine protease

99.60%

30s790241g001

36042/4.87

31000/6.48

4.98%

1

ns

−1.86

−1.99

611

Cysteine protease

99.40%

30s790241g001

36042/4.87

29000/5.15

4.98%

1

ns

ns

−2.35

07

Transporters

         

99

Vacuolar ATP synthase subunit v- proton pump b v-ATPase 57 kDa

100.00%

30s735571g002, 30s837971g002

58290/5.43

58000/5.9

3.08%

2

ns

2.13

ns

312

ATP synthase β chain

100.00%

30s884401g004

55268/5.31

54000/5.25

5.10%

2

ns

−2.28

−1.65

419

ATP synthase β chain

100.00%

30s884401g004

55268/5.31

55000/5.5

7.25%

3

ns

2.78

ns

507

ATP synthase β chain

99.50%

30s884401g004

55268/5.31

43000/5.3

2.75%

1

ns

3.96

9.99

403

CMP-sialic acid

99.90%

30s808171g003

23646/9.71

55000/4.3

9.52%

1

ns

2.52

3.23

468

Mitochondrial deoxynucleotide carrier, putative

99.70%

30s656531g003

36808/9.09

16000/5.35

2.69%

1

ns

−2.26

−2.47

09

Cell structure

         

3

Fibrillin-like protein

98.80%

30s724791g001, 30s837971g003

39091/9.45

22000/6.55

4.19%

1

ns

−2.48

−1.80

19

Plastid-lipid associated protein 3

98.30%

30s761251g002

20316/6.28

37000/6.8

5.35%

1

ns

−1.61

−2.37

53

Fibrillin-like protein

99.20%

30s724791g001, 30s837971g003

39091/9.45

21000/6.6

4.19%

1

−1.84

−1.79

1.55

317

Actin

100.00%

30s1070141g012, 30s717671g011, 30s844721g006, 30s951221g005

41610/5.31

45000/5.6

22.30%

3

ns

ns

−1.56

356

Tubulin β-2

100.00%

30s837411g003, 30s965611g001

50234/4.72

34000/5.2

9.60%

1

ns

ns

2.39

356

Tubulin β-chain

100.00%

30s1088541g002, 30s697331g005, 30s708731g001

50095/4.77

34000/5.2

9.60%

1

ns

ns

2.39

10

Signal transduction

34

Germin-like protein

98.10%

30s1014231g001

13337/5.63

18000/4.5

17.60%

1

2.71

5.01

4.50

96

Nucleoside diphosphate kinase

100.00%

30s835621g002

12145/9.0

16000/4.9

28.80%

3

1.74

−1.50

−1.62

314

IN2-1 protein

99.80%

30s696261g003

37485/6.46

19000/6.0

2.73%

1

−1.83

ns

6.10

587

Zinc finger

100.00%

30s796661g001

41956/5.55

35000/5.8

5.15%

2

−1.85

−8.66

−6.63

11

Disease/defence

         

13

Leucine rich repeat-containing

100.00%

30s857351g006

49345/4.81

64000/6

9.64%

3

ns

ns

−1.95

32

Heat shock protein 70

100.00%

30s941391g004

71057/5.13

82000/5.95

10.90%

3

ns

ns

−2.52

81

Glutathione transferase

100.00%

30s806101g003

37154/5.57

30000/5.4

8.00%

2

ns

ns

1.67

239

Heat shock protein 70

100.00%

30s795061g002

71222/5.17

70000/5.8

19.40%

1

ns

ns

−1.62

239

Heat shock protein 70

100.00%

30s941391g005

61596/5.11

70000/5.8

18.10%

3

ns

ns

−1.62

239

Heat shock protein 70

100.00%

30s941391g004

71057/5.13

70000/5.8

15.10%

1

ns

ns

−1.62

239

Heat shock cognate 70 kDa expressed

99.80%

30s1034811g003

13813/4.45

70000/5.8

17.60%

1

ns

ns

−1.62

246

ABA-hypersensitive germination 2 nucleic acid binding ribonuclease

98.70%

30s1120391g003

79218/5.98

22000/6.37

1.44%

1

ns

−1.50

−2.15

274

Aldo/keto reductase

100.00%

30s1183861g001

38732/5.97

65000/4.75

10.50%

3

ns

ns

3.30

357

ATGLX1 (glyoxalase i homolog)

99.70%

30s1166451g001

16766/5.94

24000/5.78

8.97%

1

ns

1.75

1.91

362

Heat shock protein 70

100.00%

30s941391g005

61596/5.11

70000/5.9

5.90%

2

ns

1.83

−3.04

362

Heat shock protein 70

100.00%

30s795061g002

71222/5.17

70000/5.9

6.02%

3

ns

1.83

−3.04

378

Aldo/keto reductase

99.80%

30s1183861g001

38732/5.97

65000/4.85

2.27%

1

ns

ns

3.09

403

Catalase

100.00%

30s1080231g005

55425/6.62

55000/4.3

10.70%

5

ns

2.52

3.23

404

Catalase

100.00%

30s1080231g005

55425/6.62

55000/4.25

4.62%

2

ns

3.79

3.82

503

Glutathione s-transferase GSTF2

100.00%

30s987821g001

13597/6.89

19000/4.7

14.90%

2

2.27

2.55

2.52

511

Heat shock protein 70

99.80%

30s941391g005

61596/5.11

70000/5.85

3.76%

1

ns

2.18

−2.14

523

Aldo/keto reductase

100.00%

30s1183861g001

38732/5.97

43000/5.45

11.90%

3

ns

1.84

2.64

525

Aldo/keto reductase

100.00%

30s1183861g001

38732/5.97

39000/5.34

9.07%

2

ns

3.98

5.12

583

E3 ubiquitin ligase

99.70%

30s1065691g004

121028/6.79

46000/6.25

0.91%

1

ns

−5.11

−6.36

20

Secondary metabolism

         

63

Isopentenyl diphosphate isomerase

100.00%

30s802901g001

32219/7.16

20000/5.8

6.86%

2

1.75

ns

−2.50

63

Isopentenyl pyrophosphate isomerase

100.00%

30s762371g001

17552/5.91

20000/5.8

13.10%

2

1.75

ns

−2.50

91

Chalcone isomerase

100.00%

30s1070141g002

24742/5.29

19000/5.5

4.27%

1

2.09

−1.58

−2.61

272

Chalcone isomerase

100.00%

30s1070141g002

24742/5.29

20000/5.7

24.80%

6

ns

−5.09

−4.71

274

Chalcone isomerase

100.00%

30s1070141g002

24742/5.29

65000/4.75

14.50%

3

ns

ns

3.30

286

Anthocyanidin synthase

100.00%

30s1125611g001

38960/5.99

23000/4.7

5.51%

4

−1.63

−2.44

−1.62

  1. Abbreviations: ns, not significant; NUP, no. of unique peptides.