Table 2 Differentially regulated proteins in the date hypanthium showing contrasting transient patterns compared to other fruits
From: Dynamic changes in the date palm fruit proteome during development and ripening
Spot no. | Identified protein | Protein probability | Accession no. | Theoretical MW (Da)/pI | Observed MW (Da) /pI | Sequence coverage | NUP | FC S1/MD | FC S1/NTR | FC S1/R | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
01 | Metabolism | |||||||||||
91 | Isoamyl acetate-hydrolyzing esterase | 100.00% | 30s696051g001 | 36883/5.86 | 19000/5.5 | 4.79% | 2 | 2.09 | −1.58 | −2.61 | ||
192 | Glucan endo-1,4-β-glucosidase | 100.00% | 30s896091g001 | 16069/6.54 | 60000/6.1 | 14.50% | 3 | −1.90 | −2.96 | −15.84 | ||
196 | Cytochrome p450 | 99.70% | 30s6550951g018 | 24174/4.39 | 23000/6.1 | 7.37% | 1 | ns | ns | −2.09 | ||
202 | Transketolase 1 | 99.00% | 30s656601g005 | 84589/5.66 | 95000/4.9 | 1.42% | 1 | 1.86 | −8.33 | −8.45 | ||
312 | UDP-glucose pyrophosphorylase | 100.00% | 30s904561g011 | 34207/6.1 | 54000/5.25 | 7.47% | 2 | ns | −2.28 | −1.65 | ||
317 | Sorbitol dehydrogenase | 100.00% | 30s830031g004 | 38361/5.53 | 45000/5.6 | 21.30% | 7 | ns | ns | −1.56 | ||
443 | S-adenosylmethionine synthetase | 100.00% | 30s1183641g001 | 43368/5.68 | 43000/5.1 | 11.20% | 3 | ns | −2.25 | −4.48 | ||
443 | S-adenosylmethionine synthetase | 100.00% | 30s1207141g006 | 43344/5.76 | 43000/5.1 | 8.08% | 1 | ns | −2.25 | −4.48 | ||
471 | Aldehyde dehydrogenase | 100.00% | 30s693451g001 | 59067/6.48 | 55000/4.65 | 18.80% | 11 | ns | 2.18 | −2.17 | ||
476 | S-adenosyl-l-homocysteine hydrolase | 100.00% | 30s1132381g003, 30s825931g001 | 38838/6.72 | 45000/6.0 | 13.00% | 6 | ns | ns | −2.83 | ||
476 | Aldehyde dehydrogenase | 100.00% | 30s693451g001 | 59067/6.98 | 45000/6.0 | 12.20% | 5 | ns | ns | −2.83 | ||
522 | Reversibly glycosylated polypeptide | 100.00% | 30s969461g002 | 41051/6.12 | 37000/5.9 | 3.59% | 2 | ns | −2.27 | −3.20 | ||
573 | Pyruvate decarboxylase | 99.70% | 30s919171g003 | 38883/8.66 | 60000/6.2 | 3.46% | 1 | ns | −2.33 | −5.94 | ||
660 | S-adenosylmethionine synthetase | 99.60% | 30s1183641g001, 30s1207141g006, 30s799761g004, 30s933141g001 | 43344/5.68 | 25000/6.1 | 3.28% | 1 | ns | ns | −2.64 | ||
02 | Energy | |||||||||||
2 | Pyruvate dehydrogenase E1 component β-subunit | 99.90% | 30s852391g002 | 40148/5.46 | 34000/5.74 | 6.43% | 1 | ns | −3.12 | −2.73 | ||
18 | Enolase | 100.00% | 30s663761g002 | 47828/5.91 | 55000/5.15 | 7.64% | 3 | ns | −1.90 | −1.50 | ||
403 | Citrate synthase | 99.50% | 30s1040901g002 | 30711/6.25 | 55000/4.3 | 6.64% | 1 | ns | 2.52 | 3.23 | ||
421 | Malate dehydrogenase | 100.00% | 30s892681g001 | 48314/7.11 | 49000/6.25 | 6.11% | 1 | −1.64 | −7.02 | −4.54 | ||
461 | Malate dehydrogenase | 100.00% | 30s903851g004, 30s946431g012 | 26083/8.76 | 30000/4.7 | 4.39% | 2 | ns | ns | −2.09 | ||
506 | Pyruvate dehydrogenase E1 component β-subunit | 100.00% | 30s855801g002 | 22740/5.7 | 35000/5.31 | 13.00% | 2 | ns | −1.60 | −1.70 | ||
531 | Bisphosphoglycerate-independent phosphoglycerate mutase | 100.00% | 30s661041g002, 30s693071g003 | 43543/5.55 | 30000/6.15 | 5.60% | 1 | ns | ns | 1.64 | ||
566 | Fructose-bisphosphate aldolase | 100.00% | 30s1148281g010 | 40222/7.55 | 16000/4.4 | 12.10% | 4 | ns | −2.03 | −2.99 | ||
577 | Transaldolase-like protein | 100.00% | 30s1065691g004 | 43557/5.05 | 55000/6.5 | 4.88% | 2 | −1.79 | −7.48 | −4.49 | ||
03 | Cell growth/division | |||||||||||
45 | Condensin complex subunit 1 | 99.30% | 30s702551g004 | 127265/5.78 | 51000/5.34 | 1.47% | 1 | ns | 2.53 | 1.95 | ||
252 | Enhancer of polycomb-like protein | 99.70% | 30s827291g002 | 51710/9.99 | 18000/6.2 | 5.79% | 1 | 4.51 | 4.99 | 2.11 | ||
415 | 14-3-3-like protein | 99.70% | 30s819041g002 | 17032/5.09 | 19000/6.24 | 7.95% | 1 | 2.71 | 2.55 | 1.72 | ||
04 | Transcription | |||||||||||
416 | Transcription factor IIA small subunit | 99.70% | 30s656261g002, 30s760301g006 | 12093/6.08 | 27000/4.7 | 10.40% | 1 | 2.52 | 3.51 | 2.46 | ||
05 | Protein synthesis | |||||||||||
45 | Translation initiation factor (eif-4a) | 99.90% | 30s724051g001, 30s997411g005 | 47064/5.39 | 51000/5.34 | 2.42% | 1 | ns | 2.53 | 1.95 | ||
53 | Elongation factor 1 | 99.20% | 30s667161g002 | 16163/4.26 | 21000/6.6 | 11.90% | 1 | −1.84 | −1.79 | 1.55 | ||
287 | 30s ribosomal protein s1 | 97.70% | 30s783011g009 | 45242/4.95 | 35000/6.25 | 2.15% | 1 | 1.58 | 8.73 | 11.49 | ||
416 | Peptide chain release factor, putative | 99.70% | 30s682511g003 | 9163/4.85 | 27000/4.7 | 13.30% | 1 | 2.52 | 3.51 | 2.46 | ||
504 | 60s ribosomal protein l23a | 99.70% | 30s1035671g001 | 9453/11.22 | 88000/6.0 | 11.00% | 1 | 1.62 | ns | −3.63 | ||
525 | Translation initiation factor (eif-4a) | 100.00% | 30s724051g001, 30s997411g005 | 47064/5.39 | 39000/5.34 | 9.93% | 4 | ns | 3.98 | 5.12 | ||
06 | Protein destination and storage | |||||||||||
3 | Cysteine protease | 98.80% | 30s790241g001 | 36042/4.87 | 22000/6.55 | 4.98% | 1 | ns | −2.48 | −1.80 | ||
32 | Luminal binding protein | 100.00% | 30s685511g001 | 56109/8.42 | 82000/5.95 | 7.24% | 2 | ns | ns | −2.52 | ||
92 | Mitochondrial processing peptidase | 100.00% | 30s927641g002 | 59451/5.76 | 55000/5.48 | 6.08% | 3 | ns | 1.92 | 1.88 | ||
239 | Luminal binding protein | 100.00% | 30s685511g001 | 56109/8.42 | 70000/5.8 | 18.00% | 8 | ns | ns | −1.62 | ||
288 | Cysteine protease | 99.70% | 30s790241g001 | 36042/4.87 | 32000/6.3 | 4.98% | 1 | ns | 2.43 | 2.24 | ||
303 | Subtilisin-like serine proteinase | 100.00% | 30s808251g002 | 48672/4.79 | 82000/5.9 | 5.76% | 2 | ns | −2.50 | −1.57 | ||
419 | Mitochondrial processing peptidase | 100.00% | 30s927641g002 | 59451/5.76 | 55000/5.5 | 4.60% | 1 | ns | 2.78 | ns | ||
609 | Cysteine protease | 99.60% | 30s790241g001 | 36042/4.87 | 31000/6.48 | 4.98% | 1 | ns | −1.86 | −1.99 | ||
611 | Cysteine protease | 99.40% | 30s790241g001 | 36042/4.87 | 29000/5.15 | 4.98% | 1 | ns | ns | −2.35 | ||
07 | Transporters | |||||||||||
99 | Vacuolar ATP synthase subunit v- proton pump b v-ATPase 57 kDa | 100.00% | 30s735571g002, 30s837971g002 | 58290/5.43 | 58000/5.9 | 3.08% | 2 | ns | 2.13 | ns | ||
312 | ATP synthase β chain | 100.00% | 30s884401g004 | 55268/5.31 | 54000/5.25 | 5.10% | 2 | ns | −2.28 | −1.65 | ||
419 | ATP synthase β chain | 100.00% | 30s884401g004 | 55268/5.31 | 55000/5.5 | 7.25% | 3 | ns | 2.78 | ns | ||
507 | ATP synthase β chain | 99.50% | 30s884401g004 | 55268/5.31 | 43000/5.3 | 2.75% | 1 | ns | 3.96 | 9.99 | ||
403 | CMP-sialic acid | 99.90% | 30s808171g003 | 23646/9.71 | 55000/4.3 | 9.52% | 1 | ns | 2.52 | 3.23 | ||
468 | Mitochondrial deoxynucleotide carrier, putative | 99.70% | 30s656531g003 | 36808/9.09 | 16000/5.35 | 2.69% | 1 | ns | −2.26 | −2.47 | ||
09 | Cell structure | |||||||||||
3 | Fibrillin-like protein | 98.80% | 30s724791g001, 30s837971g003 | 39091/9.45 | 22000/6.55 | 4.19% | 1 | ns | −2.48 | −1.80 | ||
19 | Plastid-lipid associated protein 3 | 98.30% | 30s761251g002 | 20316/6.28 | 37000/6.8 | 5.35% | 1 | ns | −1.61 | −2.37 | ||
53 | Fibrillin-like protein | 99.20% | 30s724791g001, 30s837971g003 | 39091/9.45 | 21000/6.6 | 4.19% | 1 | −1.84 | −1.79 | 1.55 | ||
317 | Actin | 100.00% | 30s1070141g012, 30s717671g011, 30s844721g006, 30s951221g005 | 41610/5.31 | 45000/5.6 | 22.30% | 3 | ns | ns | −1.56 | ||
356 | Tubulin β-2 | 100.00% | 30s837411g003, 30s965611g001 | 50234/4.72 | 34000/5.2 | 9.60% | 1 | ns | ns | 2.39 | ||
356 | Tubulin β-chain | 100.00% | 30s1088541g002, 30s697331g005, 30s708731g001 | 50095/4.77 | 34000/5.2 | 9.60% | 1 | ns | ns | 2.39 | ||
10 | Signal transduction | |||||||||||
34 | Germin-like protein | 98.10% | 30s1014231g001 | 13337/5.63 | 18000/4.5 | 17.60% | 1 | 2.71 | 5.01 | 4.50 | ||
96 | Nucleoside diphosphate kinase | 100.00% | 30s835621g002 | 12145/9.0 | 16000/4.9 | 28.80% | 3 | 1.74 | −1.50 | −1.62 | ||
314 | IN2-1 protein | 99.80% | 30s696261g003 | 37485/6.46 | 19000/6.0 | 2.73% | 1 | −1.83 | ns | 6.10 | ||
587 | Zinc finger | 100.00% | 30s796661g001 | 41956/5.55 | 35000/5.8 | 5.15% | 2 | −1.85 | −8.66 | −6.63 | ||
11 | Disease/defence | |||||||||||
13 | Leucine rich repeat-containing | 100.00% | 30s857351g006 | 49345/4.81 | 64000/6 | 9.64% | 3 | ns | ns | −1.95 | ||
32 | Heat shock protein 70 | 100.00% | 30s941391g004 | 71057/5.13 | 82000/5.95 | 10.90% | 3 | ns | ns | −2.52 | ||
81 | Glutathione transferase | 100.00% | 30s806101g003 | 37154/5.57 | 30000/5.4 | 8.00% | 2 | ns | ns | 1.67 | ||
239 | Heat shock protein 70 | 100.00% | 30s795061g002 | 71222/5.17 | 70000/5.8 | 19.40% | 1 | ns | ns | −1.62 | ||
239 | Heat shock protein 70 | 100.00% | 30s941391g005 | 61596/5.11 | 70000/5.8 | 18.10% | 3 | ns | ns | −1.62 | ||
239 | Heat shock protein 70 | 100.00% | 30s941391g004 | 71057/5.13 | 70000/5.8 | 15.10% | 1 | ns | ns | −1.62 | ||
239 | Heat shock cognate 70 kDa expressed | 99.80% | 30s1034811g003 | 13813/4.45 | 70000/5.8 | 17.60% | 1 | ns | ns | −1.62 | ||
246 | ABA-hypersensitive germination 2 nucleic acid binding ribonuclease | 98.70% | 30s1120391g003 | 79218/5.98 | 22000/6.37 | 1.44% | 1 | ns | −1.50 | −2.15 | ||
274 | Aldo/keto reductase | 100.00% | 30s1183861g001 | 38732/5.97 | 65000/4.75 | 10.50% | 3 | ns | ns | 3.30 | ||
357 | ATGLX1 (glyoxalase i homolog) | 99.70% | 30s1166451g001 | 16766/5.94 | 24000/5.78 | 8.97% | 1 | ns | 1.75 | 1.91 | ||
362 | Heat shock protein 70 | 100.00% | 30s941391g005 | 61596/5.11 | 70000/5.9 | 5.90% | 2 | ns | 1.83 | −3.04 | ||
362 | Heat shock protein 70 | 100.00% | 30s795061g002 | 71222/5.17 | 70000/5.9 | 6.02% | 3 | ns | 1.83 | −3.04 | ||
378 | Aldo/keto reductase | 99.80% | 30s1183861g001 | 38732/5.97 | 65000/4.85 | 2.27% | 1 | ns | ns | 3.09 | ||
403 | Catalase | 100.00% | 30s1080231g005 | 55425/6.62 | 55000/4.3 | 10.70% | 5 | ns | 2.52 | 3.23 | ||
404 | Catalase | 100.00% | 30s1080231g005 | 55425/6.62 | 55000/4.25 | 4.62% | 2 | ns | 3.79 | 3.82 | ||
503 | Glutathione s-transferase GSTF2 | 100.00% | 30s987821g001 | 13597/6.89 | 19000/4.7 | 14.90% | 2 | 2.27 | 2.55 | 2.52 | ||
511 | Heat shock protein 70 | 99.80% | 30s941391g005 | 61596/5.11 | 70000/5.85 | 3.76% | 1 | ns | 2.18 | −2.14 | ||
523 | Aldo/keto reductase | 100.00% | 30s1183861g001 | 38732/5.97 | 43000/5.45 | 11.90% | 3 | ns | 1.84 | 2.64 | ||
525 | Aldo/keto reductase | 100.00% | 30s1183861g001 | 38732/5.97 | 39000/5.34 | 9.07% | 2 | ns | 3.98 | 5.12 | ||
583 | E3 ubiquitin ligase | 99.70% | 30s1065691g004 | 121028/6.79 | 46000/6.25 | 0.91% | 1 | ns | −5.11 | −6.36 | ||
20 | Secondary metabolism | |||||||||||
63 | Isopentenyl diphosphate isomerase | 100.00% | 30s802901g001 | 32219/7.16 | 20000/5.8 | 6.86% | 2 | 1.75 | ns | −2.50 | ||
63 | Isopentenyl pyrophosphate isomerase | 100.00% | 30s762371g001 | 17552/5.91 | 20000/5.8 | 13.10% | 2 | 1.75 | ns | −2.50 | ||
91 | Chalcone isomerase | 100.00% | 30s1070141g002 | 24742/5.29 | 19000/5.5 | 4.27% | 1 | 2.09 | −1.58 | −2.61 | ||
272 | Chalcone isomerase | 100.00% | 30s1070141g002 | 24742/5.29 | 20000/5.7 | 24.80% | 6 | ns | −5.09 | −4.71 | ||
274 | Chalcone isomerase | 100.00% | 30s1070141g002 | 24742/5.29 | 65000/4.75 | 14.50% | 3 | ns | ns | 3.30 | ||
286 | Anthocyanidin synthase | 100.00% | 30s1125611g001 | 38960/5.99 | 23000/4.7 | 5.51% | 4 | −1.63 | −2.44 | −1.62 | ||