Figure 5
RT-qPCR of analysis of gene expression in ‘Su Shuai’ and ‘Indo’, relative to ‘Golden Delicious’. Specific genes were selected based on their associations with different horticultural traits (disease resistance, internode length and fruit flavor). All RT-qPCR analyses were repeated three times (biological replicates). Apple Tubulin, Mdtubulin was used as a housekeeping gene (GenBank accession number AJ421411). The x-axis indicates the different genotypes. The y-axis shows the relative expression level as determined by RT-qPCR. The level of gene expression in ‘Golden Delicious’ was arbitrarily assigned a value of 1.0.
