Table 10 Sequences of gene-specific primers used in RT-qPCR analysis of different genes
Gene | Primer name | Sequence 5′–3′ | Usage | Size (bp) | Gene IDa |
|---|---|---|---|---|---|
MbR7 | MbR7.F | TCT CAG TCT CAT CAT TGG AAG | Expression of MbR7 homolog | 196 | MDP0000233155 |
MbR7.R | AGA GGC GTG TAA GAG GAT | ||||
N | N.F | AGC CTT CCA AGC CTC AGT | Expression of N homolog | 121 | MDP0000272916 |
N.R | AGCAGTC GTC CAT CTC CAG | ||||
GA3OX1 | GA3OX1.F | T GTC CAT TGT CCA CCA GAG | Expression of GA3OX1 homolog | 106 | MDP0000233590 |
GA3OX1.R | C CAA CAT TGA CCA CCA GAG | ||||
RGL2 | RGL2.F | A AGG AAA CAC TTG AAT GGC | Expression of RGL2 homolog | 127 | MDP0000300311 |
RGL2.R | C ACT GCG GTT ATT GTA AGG | ||||
GA-neg | GA-neg.F | ACT TCA CCT CAG CGT CTC | Expression of GA-neg homolog | 133 | MDP0000284679 |
GA-neg.R | TGC CTC GTG CTA TAT CTA TCG | ||||
SDH | SDH.F | T GGT CAT TCA GAG CAC TAC TTC | Expression of GA-reg homolog | 124 | MDP0000693768 |
SDH.R | AAC TGT TGT CAA GGT CAC GAA | ||||
CINV2 | CINV2.F | AGA GGT AGG TTC ATT GGA | Expression of CINV2 homolog | 179 | MDP0000745777 |
CINV2.R | CGC TTT CTT CTT GGG TTA | ||||
NAD-ME2 | NAD-ME2.F | A CAG ATG GCA GTC GTA TTC | Expression of NAD-ME2 homolog | 186 | MDP0000453139 |
NAD-ME.R | A GCA AAG TCA CTC AAT GGT | ||||
Mdtubulin | Mdtubulin.F | AG GAT GCT ACA GCC GAT GAG | Housekeeping gene | 192 | AJ421411b |
Mdtubulin.R | GCC GAA GAA CTG ACG AGA ATC | Genbank accession No. |
