Figure 1
From: Dynamic changes in proteins during apple (Malus x domestica) fruit ripening and storage
Identification and differential expression of apple (cv. Golden Delicious) proteins in fruit maintained at 25 °C and sampled during ripening and senescence. 2-DE was performed using 1.8 mg of protein, linear 17 cm IPG strips (pH 4–7) for the first dimension, and 12% SDS–PAGE gels for the second dimension electrophoresis. Gels were stained with colloidal CBB G250. Numbers with arrows indicate the differentially expressed protein spots that were identified in this study. CBB, Coomassie brilliant blue; IEF, isoelectric focusing; IPG, immobilized pH gradient; SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis.
