Table 3 NPA promotes cytokinin-mediated shoot organogenesis in ‘Carrizo’ citrange+

From: Endogenous auxin and its manipulation influence in vitro shoot organogenesis of citrus epicotyl explants

Cytokinin (BAP) (mg L-1)

NPA (20 mg L-1)

Apical-end organogenesis rate ++

Basal-end organogenesis rate ++

Total organogenesis rate ++

0

−

1.04±0.04c

0.07±0.07a

1.11±0.11c

0

+

1.14±0.10c

0

1.14±0.10c

0.5

−

2.24±0.12e

0.65±0.30b

2.89±0.41f

0.5

+

2.74±0.21f

1.03±0.03c

3.76±0.18g

1

−

2.15±0.15e

1.15±0.15c

3.30±0.01f,g

1

+

3.03±0.50f

1.68±0.03d

4.76±0.48h

3

−

2.38±0.16e

1.55±0.32d

3.93±0.17g

3

+

3.87±0.19g

2.61±0.05f

6.47±0.18i

  1. + NPA is an auxin transport inhibitor. The fact that NPA’s promotional effects on shoot organogenesis are cytokinin-dependent indicates auxin represses the cytokinin-mediated shoot organogenesis.
  2. ++ Organogenesis rate was calculated by dividing the total shoot number by the total explant number.
  3. Values followed by the different letters are significantly different at P<0.05 (ANOVA; LSD).
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