Table 2 Primers, probes and other PCR conditions for genotyping

From: Association of polymorphisms of SORBS1, GCK and WISP1 with hypertension in community-dwelling Japanese individuals

Gene

Polymorphism

Sense primer

Antisense primer

Probe with Texas red

Mg 2+ (mmol/l)

SORBS1

682A → G (Thr228Ala)

ATTTCATTCCCCCTCATCTGCAGAG

ACCAGCGGAGGTGGTGGTGAG

ATGGTGGCGCCTGGCTAAT

2.0

GCK

−30G → A

CTCCTGGTCACCATGACAACCACAG

TGCTCCAGCCAGGTGTGGAGTG

CCTCTCAGGAGCACAGTAAGC

2.0

WISP1

2364A → G

CAAATGGCCAGTTTTCTGGTAGGAAG

TTCAACCTCTTCAGCTTTAAACCTTTATTAAGTC

GGAGGTTTACCGGTTGTTTAGA

3.0

  1. Oligonucleotide sequences are 5′ → 3′.
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