Figure 2

Effect of antihypertensive drugs on CTGF distribution in rat PO hearts. (a) Saline was administered to the PO heart for 28 days, using an osmotic pump. Considerable distribution of CTGF-positive signals (red) was observed around the perivascular area and interstitial fibrotic area. The dotted boxed area shows the area that was observed at higher magnification in (d). (b) Hydralazine was administered to the PO heart for 28 days, using an osmotic pump. CTGF-positive signals (red) were observed around the perivascular area and interstitial space. The dotted boxed area shows the area observed at higher magnification in (e). (c) Olmesartan was administered to the PO heart for 28 days, using an osmotic pump. CTGF-positive signals (red) were faint around the perivascular area and interstitial space. The dotted boxed area shows the area observed at higher magnification in (f). (d) Higher magnification of the vascular area (boxed region) in A. CTGF-positive signals (red) was distinctly observed around the vessels and interstitial space. (e) Higher magnification of the vascular area (boxed region) in (b). CTGF-positive moderate signals (red) were observed around the vessels and interstitial space. (f) Higher magnification of the vascular area (boxed region) in (c). CTGF-positive moderate signals (red) were faint around the vessels and interstitial space. Scale bar: 100 μm.