Figure 1
Expression of N-type calcium channels in H295R cells. (a) Inhibitory effect of 100 nM omega-conotoxin GVIA (CnTX) on voltage-dependent calcium channel (VDCC) currents in H295R cells. The representative traces for barium currents upon application of 30-ms test pulses from −50 to 50 mV with 10-mV increments starting from a Vh of −80 mV. (b) Current density–voltage (I–V) relationships of VDCCs. I–V relationships in control (non-treat), 100 nM CnTX-treated conditions (CnTX), and subtraction of barium currents in the presence of CnTX from control barium current (subtracted). Data were obtained from three independent experiments (n=9) and expressed as the mean±s.e.m. (c) mRNA expression of the N-type calcium channel in H295R cells and human tissue. The data represent calcium channel mRNA expression in human tissues and cells (mean). (d) Western blotting of N-type calcium channel in H295R cells. Total protein (600 μg) was loaded. The CaV2.2 immunoreactivity is identified by incubation with anti-CaV2.2 (1:200). Identical procedures except the anti-CaV2.2 subjected to peptide block (anti-CaV2.2+peptide) fail to detect specific immunoreactivity.
