Figure 3

Expression of N-type calcium channels in H295R cells and the functional role of these channels in angiotensin II (Ang II)-induced aldosterone production. (a) Intracellular Ca²⁺ level as a cellular response to Ang II stimulation (0.1–10 μM). (b) The peak intracellular Ca²⁺ level was dependent on Ang II concentration. The data represent the percentage values of the intensity of fluorescence in the stimulated cells, and these values were relative to the corresponding values in cells treated with 100 nM of Ang II. (c) Inhibitory effect of omega-conotoxin GVIA (CnTX) on Ang II-induced intracellular Ca²⁺ increment in H295R cells. The data represent the percentage values of the intensity of fluorescence in the incubated cells; these values were relative to the corresponding values for cells treated with 100 nM of Ang II. All the results are shown as the mean±s.e.m. (n=5–8). ^†††P<0.001 for non-treated H295R cells vs. control. ^*P<0.05 for the control group vs. each CnTX-treatment group.