Figure 4

Inhibitory effect of N-type calcium channel blockers on corticosteroid production in H295R cells stimulated with 100 nM angiotensin II (Ang II). (a) Inhibitory effect of omega-conotoxin GVIA (CnTX) on aldosterone production in H295R cells stimulated with 100 nM Ang II. The bars represent the percentage values of the aldosterone secretion of culture supernatants (n=15). These values were normalized by the cellular protein content and were relative to the corresponding values for cells treated with 100 nM of Ang II (control; 19±2 pg/μg protein). (b) Inhibitory effect of CnTX on cortisol production in H295R cells stimulated with 100 nM Ang II. The bars represent the percentage values for cortisol secretion by the culture supernatant (n=8), and these values were normalized by the cellular protein content and were relative to the corresponding value for the cells treated with 100 nM Ang II (control; 2255±352 pg/μg protein). (c) Effect of 100 nM CnTX on the expression of CYP11B2 after stimulation with 100 nM Ang II (n=4). (d) Effect of 100 nM CnTX on the expression of CYP11B1 after stimulation with 100 nM Ang II (n=4). ^†††P<0.001 and ^†P<0.05 for the non-treated group vs. the control group. All results are shown as means±s.e.m. ^*P<0.05; ^**P<0.01; and ^***P<0.001 for the control group vs. each CnTX-treatment group.