Figure 5

Effect of α1B knockdown on corticosteroid production and gene expression in H295R cells stimulated with 100 nM angiotensin II (Ang II). (a) In comparison with non-target small interfering RNA (siRNA), α1B siRNA caused a significant reduction in α1B mRNA expression during the entire investigation. These values were relative to the corresponding value for the cells treated with non-target siRNA (n=4). (b) The effect of α1B RNA interference on Ang II-induced aldosterone production in H295R cells. The increase in aldosterone production was significantly inhibited by the siRNA for the N-type calcium channel. The bars represent the percentage values normalized by the cellular protein content, and these values were relative to the corresponding value for the cells treated with non-target siRNA (n=5). (c) The effect of α1B RNA interference on the expression of CYP11B2 after stimulation with 100 nM Ang II (n=8). These values were relative to the corresponding value for the cells treated with non-target siRNA (n=8). (d) The effect of α1B RNA interference on the expression of CYP11B2 after stimulation with 100 nM Ang II (n=8). These values were relative to the corresponding value for the cells treated with non-target siRNA (n=8). ^†††P<0.001, and ^††P<0.001 for the difference among the siRNA-non-treated groups. All results are shown as means±s.e.m. ^###P<0.001, ^##P<0.001, and ^##P<0.001 for the difference among the siRNA-treated groups.