Figure 6

Inhibitory effect of each CCB on corticosteroid production and gene expression in H295R cells stimulated with 100 nM Ang II. (a) Each CCB has a different action on aldosterone secretion. The bars represent the percentage values normalized by the cellular protein content (n=12). These values were relative to the corresponding value for the cells treated with 100 nM angiotensin II (Ang II; control; 18±3 pg/μg protein). (b) Each CCB has a different action on cortisol secretion, and this action depends on the type of the blocked calcium channel. The bars represent the percentage values normalized by the cellular protein content (n=8), and these values were relative to the corresponding value for the cells treated with 100 nM Ang II (control; 2255±352 pg/μg protein). (c) The effect of each CCB (100 nM) on the expression of CYP11B2 after stimulation with 100 nM Ang II (n=4). The bars represent the percentages of CYP11B2 mRNA expression, which were normalized and were relative to the corresponding value for the control (Ang II, 100 nM). (d) The effect of each CCB (100 nM) on the expression of CYP11B1 after stimulation with 100 nM Ang II (n=4). The bars represent the percentages of CYP11B1 mRNA expression, which were normalized and were relative to the corresponding value for the control (Ang II, 100 nM). ^†††P<0.001 for the non-treated group vs. the control group. ^*P<0.05; ^**P<0.01; and ^***P<0.001 for the control group vs. each CCB group. CCB, calcium channel blocker; Cil, cilnidipine; Efo, efonidipine; Nif, nifedipine.