Figure 1 | Hypertension Research

Figure 1

From: Inhibition of farnesyl pyrophosphate synthase prevents norepinephrine–induced fibrotic responses in vascular smooth muscle cells from spontaneously hypertensive rats

Figure 1

NE exerts greater effects on proliferation, CTGF and ECM upregulation in cultured VSMCs from SHR than WKY. Quiescent VSMCs from SHR and WKY were incubated with NE (0.01, 0.1, 1, 10 μM) for 24 h. (a) shows cell proliferation expressed as the optical density which was determined by Cell Counting Kit (CCK-8). (b) shows levels of CTGF mRNAs as determined by RT-qPCR analysis. The ratio of CTGF mRNAs is set to ‘1’ for WKY control group. (c) shows in top panel a representative western blot of CTGF protein expression and in bottom data bar chart. The ratio of CTGF protein is set to ‘1’ for WKY control group. (d) shows levels of type I pro-collagen mRNAs as determined by RT-qPCR analysis. The ratio of α-1 procollagen I mRNAs is set to ‘1’ for WKY control group. (e) shows Hydroxyproline (Hyp) levels (as an index of collagen) in the culture supernatant as measured with the Hyp test kit. The level of hydroxyproline is set to ‘1’ for WKY control group. Data are expressed as mean±s.e.m. (n=5). #P<0.05, ##P<0.01 vs corresponding WKY group; *P<0.05, **P<0.01 vs corresponding control group.

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