Figure 1

Changes in adiponectin and interleukin-6 (IL-6) production during adipogenesis. 3T3-L1 preadipocytes were induced to differentiate into adipocytes. The messenger RNA (mRNA) expression of (a) adiponectin and (c) IL-6 assessed by quantitative reverse transcriptase PCR and the concentrations in the culture medium of (b) adiponectin and (d) IL-6 by enzyme-linked immunosorbent assay were compared between preadipocytes immediately before induction (D0) and mature adipocytes at the seventh day of adipogenesis (D7). The mRNA expression was normalized to that of cyclophilin B. n=3. *P<0.05 vs. D0 by Student’s t-test.