Figure 3 | Hypertension Research

Figure 3

From: Valsartan ameliorates the constitutive adipokine expression pattern in mature adipocytes: a role for inverse agonism of the angiotensin II type 1 receptor in obesity

Figure 3

Effects of angiotensin II type 1 receptor (AT1R) and AT2R on basal and valsartan-induced interleukin-6 (IL-6) expression. 3T3-L1 adipocytes 8 days after adipogenic induction were treated with dimethyl sulfoxide (Cont), 50 μmol l−1 valsartan (Val), 10 μmol l−1 PD123319 alone (PD) or a combination (PD+Val) for 2 days. (a) The messenger RNA (mRNA) expression and (b) release in the culture medium of IL-6 were assessed by quantitative reverse transcriptase PCR and enzyme-linked immunosorbent assay, respectively. The mRNA expression was normalized to that of cyclophilin B. n=3. *P<0.05 vs. Cont, #P<0.05 vs. Val, †P<0.05 vs. PD (analysis of variance followed by a Tukey’s post hoc test). Cont, control.

Back to article page