Figure 5
Effects of valsartan on constitutive IκB-α activity for interleukin-6 (IL-6) expression and on nuclear NF-κB1 protein level. 3T3-L1 adipocytes 8 days after adipogenic induction were treated with dimethyl sulfoxide (Cont), 50 μmol l−1 valsartan (Val), 10 μmol l−1 MG132 alone (MG) or a combination (MG+Val) for (a, b) 1 day or (c) for 2 days. (a) Protein levels of IκB-α and the loading control, β-tubulin, in the cytoplasmic extract; and (b) those of NF-κB1 and Lamin A in the nuclear extract were assessed by western blot. Representative blots are shown, and the graph shows the results of a densitometric quantification in which (a) IκB-α and (b) NF-κB1 protein levels were normalized to those of β-tubulin and Lamin A, respectively. (c) The mRNA expression of IL-6 was assessed by quantitative reverse transcriptase PCR. n=3. *P<0.05 vs. Cont, #P<0.05 vs. Val, †P<0.05 vs. MG (analysis of variance followed by a Tukey’s post hoc test). Cont, control.
