Figure 6

Effects of valsartan on peroxisome proliferator-activated receptor (PPAR)-γ target molecules in mature adipocytes. 3T3-L1 adipocytes 8 days after adipogenic induction were treated with dimethyl sulfoxide (Cont) or 50 μmol l−1 valsartan (Val) for 2 days. Messenger RNA (mRNA) expression of (a) PPAR-γ, (b) PGC-1α, (c) phosphoenolpyruvate carboxykinase (PEPCK) and (d) adiponectin was assessed by quantitative reverse transcriptase PCR. mRNA expression was normalized to that of cyclophilin B. (e) The release of adiponectin in the culture medium was assessed by enzyme-linked immunosorbent assay. n=3. *P<0.05 vs. Cont by Student’s t-test. Cont, control.