Figure 4

Renal NOX4 and AT1R protein expression in hD5^WT and hD5^F173L transgenic mice. (a) Renal NOX4 protein expression (67 kDa) determined by immunoblotting in hD5^WT and hD5^F173L transgenic mice fed normal salt diet (n=6, *P<0.05 vs. hD5^WT, t-test). (b) Renal NOX4 protein expression (67 kDa) determined by immunoblotting in hD5^F173L transgenic mice on normal or high salt diet (n=6, *P<0.05 vs. normal salt, t-test). (c) AT1R expression (43 kDa) determined by immunoblotting in hD5^WT and hD5^F173L mice on normal salt diet (n=6, *P<0.05 vs. hD5^WT, t-test). All immunoblotting results are expressed as relative DUs. Immunoblots of NOX4, AT1R, and GAPDH are depicted in the inset. DU, density unit.