Table 1 Primers designed for real-time PCR

From: Continuous infusion of angiotensin II modulates hypertrophic differentiation and apoptosis of chondrocytes in cartilage formation in a fracture model mouse

Name

Sense primer (5′-3′)

Antiense primer (5′-3′)

Sox9

GAGCCGGATCTGAAGATGGA

GCTTGACGTGTGGCTTGTTC

Runx2

AGATGATGACACTGCCACCTCTG

GGGATGAAATGCTTGGGAACTGC

Type X collagen

TCTGGGATGCCGCTTGT

CGTAGGCGTGCCGTTCTT

MMP-13

TGATGAAACCTGGACAAGCA

CCTGGGTCCTTGGAGTGAT

Ihh

TGGACTCATTGCCTCCCAGA

CAAAGGCTCAGGAGGCTGGA

bcl-2

TCGCAGAGATGTCCAGTCAG

ATGCCGGTTCAGGTACTCAG

bcl-xL

TCAGAGCTTTGAGCAGGTAGTG

TCCCGTAGAGATCCACAAAAG

Bax

TGCAGAGGATGATTGCTGAC

GATCAGCTCGGGCACTTTAG

GAPDH

ATGGTGAAGGTCGGTGTGA

CCATGTAGTTGAGGTCAATGAG

  1. Abbreviations: Bax, BCL-2-associated X protein; bcl-2, B-cell leukemia/lymphoma-2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Ihh, Indian hedgehog; MMP-13, matrix metalloproteinase-13; Runx2, runt-related transcription factor 2; Sox9, SRY-box containing gene 9.
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