Figure 3
From: Notch activation of Ca2+-sensing receptor mediates hypoxia-induced pulmonary hypertension
Downregulation of Notch3 with a small interfering RNA (siRNA) attenuates the increase in hypoxia-induced pulmonary arterial smooth muscle cell (PASMC) proliferation. The increase in hypoxia-induced PASMC proliferation was enhanced by Jag-1 and restored to normal levels by DAPT. Summarized data showing the total numbers of normal PASMCs and acute hypoxic PASMCs after culture in growth media for 48, 72 and 96 h (n=3 experiments). Data showing the inhibitory effects of the Notch3-siRNA on the proliferation of hypoxic PASMCs (**P<0.01 vs. control-siRNA; a). Summarized data showing the stimulatory effects of Jag-1 on the proliferation of normoxic PASMCs (b). Summarized data showing the inhibitory effects of DAPT on the proliferation of hypoxic PASMCs (c; **P<0.01, hypoxia+DAPT vs. hypoxia+vehicle; hypoxia+Jag-1 vs. hypoxia+vehicle).
