Figure 4 | International Journal of Obesity

Figure 4

From: Suppression of inflammation-associated factors by indole-3-carbinol in mice fed high-fat diets and in isolated, co-cultured macrophages and adipocytes

Figure 4

Effects of I3C on peroxisome proliferator-activated receptor (PPAR-γ) mRNA in co-culture of primary adipocytes and macrophages. Differentiated adipocytes were co-cultured with RAW 264.7 macrophages (1 × 106 cells per well), and cell treated with I3C for 24 h. Differentiated adipocytes were treated with ethanol (0 μM I3C), 1 μM I3C, 10 μM I3C or 100 μM I3C, as indicated. The expression of PPAR-γ mRNAs was determined by reverse transcriptase polymerase chain reaction. The total cellular RNA was isolated and reverse-transcribed to cDNA (a). The relative expression of PPAR-γ under different concentrations of I3C was summarized (b). These experiments were repeated three times. Values with different superscript letters (a and b) indicate significant differences between groups (P<0.05).

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