Figure 5 | International Journal of Obesity

Figure 5

From: Suppression of inflammation-associated factors by indole-3-carbinol in mice fed high-fat diets and in isolated, co-cultured macrophages and adipocytes

Figure 5

Effects of I3C on intracellular lipid accumulation (a) and triglyceride accumulation (TG) content (b) in 3T3-L1 adipocytes. 3T3-L1 were differentiated by culturing with adipogenic agent. Cells were treated with various concentrations of I3C for 72 h. Cells were stained with oil red O. TG contents were quantified by a commercial assay kit, as described in Materials and Methods. These experiments were repeated three times, and similar results were obtained. Control: undifferentiated 3T3-L1 preadipocytes; 0 (0 μM I3C): differentiated 3T3-L1 adipocytes treated with ethanol; 1–100 μM: differentiated adipocytes treated with 1–100 μM I3C. Values are the mean±s.d. from three measurements. Values with different superscript letters (a, b, and c) indicate significant differences between groups (P<0.05).

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