Figure 4

mRNA expression of insulin-signalling components in epididymal adipose tissue from 3-month-old control and recuperated male rats. (a) Forward (F) and reverse (R) primer sequences. (b) mRNA expression determined by quantitative reverse-transcriptase PCR and normalised to mRNA expression of housekeeping gene cyclophilin-A (Cyc-A). Data presented as mean±s.e.m.; n=6 per group.