Figure 3

Let-7g suppresses the proliferation of mesenchymal stem cells. (a) Cell proliferation rate was lower in C3H10T1/2 cells infected with lentiviral plasmids Puro.let-7g.Cre compared with C3H10T1/2 cells or C3H10T1/2 cells infected with Puro.let-7gsm.Cre. Either 2 × 10³ or 10⁴ cells were plated in 96-well plates for analysis. (b) Cell proliferation related genes, cyclin D1, CDK4 and proliferation cell nuclear antigen were decreased after let-7g overexpression. (c) C3H10T1/2 cells infected with Puro.let-7g.Cre grew slower than C3H10T1/2 cells and C3H10T1/2 cells infected with Puro.let-7gsm.Cre. Either 10⁴ or 3 × 10⁴ cells were plated in 6-well plates for analysis (*P<0.05, ^§P<0.10 when compared with let-7gsm; Mean±s.e.m.; n=4 in each group).