Figure 4

Let-7g inhibits expression of adipogenic differentiation markers and adipogenesis in C3H10T1/2 cells treated with 5′-azacytidine. (a) mRNA expression of PPARγ without or with adipogenic induction, which were both lower in cells infected with Puro.let-7g.Cre. (b) mRNA expression of CCAAT/enhancer-binding protein alpha (C/EBPα) without or with adipogenic induction, which were both lower in cells infected with Puro.let-7g.Cre. (c) Protein content of PPARγ without or with adipogenic induction, which were both lower in cells infected with Puro.let-7g.Cre. (d) Protein expression of C/EBPα without or with adipogenic induction, which were both lower in cells infected with Puro.let-7g.Cre. (e) After cultured in adipogenic differentiation medium for 10 days, there were less adipocytes formed in C3H10T1/2 cells infected with lentiviral plasmid Puro.let-7g.Cre compared with cells infected with Puro.let-7gsm.Cre plasmid (left: x1magnification; right: x100 magnification). (*P<0.05, **P<0.01 when compared with let-7gsm; mean±s.e.m.; n=4; □, cells without adipogenic induction; , cells after adipogenic differentiation).