Figure 1

Lymphocyte phenotype and activation in adipose tissue according to levels of central adiposity. (a) Relative gene expression of cluster differentiation markers to identify T lymphocytes (n=30), (b) CD4+ T lymphocyte subsets present within the adipose tissue (n=30) and (c) proportions of activated T lymphocytes in adipose tissue SVF as a percentage of CD4+ and CD8+ cells measured by flow cytometry (n=17). Gene expression data presented as mean $2^{{\text{-ΔΔC}}_{\text{t}}}$±s.e.m. Effects of adiposity were analysed by one-way analysis of variance (ANOVA), *P<0.05 and **P<0.001. (d) (i–iv) Correlations between waist circumference and activation status of CD4+ and CD8+ T lymphocytes in the adipose tissue (n=17) were analysed using Pearson’s (R) correlation; coefficients shown along with significance values. MFI, mean fluorescence intensity.