Figure 6
Effects of C12TPP on the rates of oxygen consumption and RER in wild-type mice on a HFD at thermoneutrality. (a) Change in body weight over time relative to day 0 in control, C12TPP-treated and pair-fed to treated mice. Treatment started on day 0 and finished on day 7, as is indicated by arrows. The mice were exposed twice to indirect calorimetry measurements on day 1 and 7 of treatment. The periods inside the chamber are indicated by red lines. The values are the means±s.e.m. of five to six mice per group. The effects were statistically analyzed using a repeated measures two-way analysis of variance (ANOVA) (time: P<0.01; treatment: P<0.01; interaction P<0.01). #Significant differences between the treated and pair-fed groups. Significant differences from the control are not shown. (b) Rate of oxygen consumption (ml O2 per min·g lean body mass) of treated and pair-fed mice on the seventh day of treatment. Nighttime is indicated by the black box on the x axis. The values are the means of six mice per group. (c) TEE (TEEbal) estimated using the energy balance method for the 6 days of treatment. (d) RMR in C12TPP-treated and pair-fed mice during the seventh light phase of day of treatment. In c and d, the values are the means±s.e.m. (n=6 for each group). The data were analyzed with a paired Student’s t-test and the # indicates significant differences between the pair-fed and treated groups. (e) RER traces during the seventh day of treatment. Each trace is the mean of n=6 mice per group. (f) Average RER of treated and pair-fed mice. The values are the means±s.e.m. (n=5–6 for each group). The effects were statistically analyzed with a two-way ANOVA matched for both time and mouse (separate for day 1 and day 7 due to not being the same animals): day 1 (time: P<0.05; treatment: P<0.05; interaction P<0.05); day 7 (time: P<0.05; treatment: P<0.05; interaction P<0.01). #Significant differences between the treated and pair-fed groups.
