Figure 5

Epac2a knockout results in a severer high-fat diet-induced leptin resistance. (a) Daily and (b) cumulative food intake of WT and Epac2a KO mice during the 8 weeks of high-fat diet (HFD) feeding (n=9). (c) Plasma leptin and adiponectin levels in fed WT and KO mice after 8 weeks of HFD feeding (n=9). (d) Daily food intake after saline and leptin administration (i.p. 2 mg kg⁻¹) during the indicated period in WT and KO mice after 8 weeks of HFD feeding (n=6). (e) Representative western blot of PTP1B and SOCS-3 proteins in the hypothalamus of WT and KO mice after 8 weeks of normal chow diet or HFD feeding (n=6). (f) Hypothalamic expression of Pomc and Npy genes in WT and KO mice fed a normal chow diet and HFD for 8 weeks (n=9). (g) Representative western blot of Epac2a protein in the hypothalamus of WT and KO mice after 8 weeks of HFD feeding and quantification of Epac2a western blot normalized to GAPDH (n=3). The values represent the mean±s.e.m. *P<0.05 vs WT mice in HFD.