Figure 5

A history of diet-induced obesity covers a proinflammatory hepatic transcription profile. (a) Heat map of 322 significantly (P<0.01) regulated hepatic genes in the comparison of formerly obese and lean mice (n=6). Top up- and downregulated genes are presented separately. A detailed list of the 322 differentially expressed genes is attached in Supplementary Table 6. (b) Top five statistically significant (by P-value; P<0.01) activated ‘Diseases and Bio Functions’ associated with the 322 regulated genes. A detailed list of annotated terms can be found in Supplementary Table 7. (c) Significantly activated predicted upstream regulators of the 322 regulated hepatic genes. A list of all predicted activated upstream regulators of the 322 differentially expressed genes can be found in Supplementary Table 8. (d) Venn-diagram of (overlapping) differentially expressed genes (FC>1.2 P<0.01) in three pairwise comparisons ‘formerly obese vs lean’ (left panel), ‘obese vs lean’ (upper panel) and ‘formerly obese vs obese’ (right panel). A detailed list of the differentially expressed genes regarding each comparison can be found in Supplementary Tables 6, 9–13.