Figure 1
From: Neuromodulation of orexin neurons reduces diet-induced adiposity
Cre-dependent genetic targeting and pharmacosynthetic activation of orexin neurons (N=9). (a) Schematic diagram of AAV vector encoding DREADD-mCherry driven by Elongation factor alpha (EFa1) promoter sequence and flanked by dual flox sites for recombination in the presence of Cre-recombinase. Cre is driven by the prepro-orexin-promoter of Orexin:Cre mice. (b) Schematic of anatomical targeting with stereotactic viral infusion. Photomicrographs of coronal sections containing immunofluorescent orexin neurons (green) and hM3Dq-mCherry (red; C) or cFos (red; F). Percent of orexin neurons expressing mCherry (orange arrow head) in Cre::hM3Dq (d) and Wt::hM3Dq (e) mice. Percentage of orexin neurons expressing Fos (red arrowhead) after CNO (5 mg/kg; G) or vehicle (E). DAPI (blue), nuclear label; Cre, orexin-cre; lateral hypothalamus (LH), fornix (f), dorsal medial hypothalamus (DMH), ventral medial hypothalamus (VMH); inverted terminal repeat (ITR); woodchuck post-regulatory regulatory element (WPRE).
