Figure 4 | International Journal of Obesity

Figure 4

From: Adipocytes sustain pancreatic cancer progression through a non-canonical WNT paracrine network inducing ROR2 nuclear shuttling

Figure 4

Adipocytes-derived WNT secreted factors induced expression and cellular rearrangement of ROR2 in pancreatic preneoplastic lesions. (a) Western blot analysis for the expression of ROR2 in HPDE and HPDE/KRAS cultured with ADIPOCM and hBMSCCM treated or untreated with vantictumab. GAPDH was used as loading control. (b) Immunofluorescence colocalization analysis for ROR2 (red) and FZD (green) receptors in HPDE and HPDE/KRAS cells cultured with ADIPOCM or with hBMSCCM treated or untreated with vantictumab. Fluorescence intensity analysis was performed by ImageJ software. Fluorescence intensity analysis was performed by ImageJ software and bars represent mean±s.d. from analysis of four separate high-power field images. ***P<0.001, by two-tailed unpaired Student’s t-test.

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