Figure 5 | International Journal of Obesity

Figure 5

From: Adipocytes sustain pancreatic cancer progression through a non-canonical WNT paracrine network inducing ROR2 nuclear shuttling

Figure 5

ROR2 nuclear shuttling mediates pancreatic preneoplastic lesions aggressiveness induced by adipocytes-derived WNT paracrine factors. (a) Western blot analysis for the expression of both 105 and 50 kDA protein isoforms of ROR2 in cytosolic and nuclear compartment from HPDE and HPDE/KRAS cultured with ADIPOCM and hBMSCCM treated or untreated with vantictumab. GAPDH and Histone H3 were used as loading and cellular fraction compartment controls. (b) Immunofluorescence colocalization analysis for ROR2 (green) and nucleus (blue) in HPDE and HPDE/KRAS cells cultured with ADIPOCM or with hBMSCCM treated or untreated with vantictumab. Fluorescence intensity analysis was performed by ImageJ software.

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