Figure 4 | International Journal of Obesity

Figure 4

From: Global transcriptome analysis identifies weight regain-induced activation of adaptive immune responses in white adipose tissue of mice

Figure 4

Flow cytometric analysis of immune cell population in gWAT of weight regain mouse model (a) Flow profiles of stromal vascular fraction cells isolated from gWAT of CTL, WRG, WG and WLM mice. (b) Quantification of macrophage subsets and dendritic cells. (CD64+CD11b+ population represents total macrophages. CD11c-positive cells (M1 macrophages), CD206-positive cells (M2 macrophages), MHC II and CD11c-positive cells (dendritic cells) (c) M1 to M2 ratio at 16 weeks of diet-challenge. (d) Quantification of the adaptive immune cell population, gated using CD3 and CD19, to identify T cells and B cells. CD3-positive gated cells were analyzed for Foxp3, CD4-Foxp3-positive cells (Regulatory T cells, Treg), and CD4-positive cells (CD4+ T cells) were analyzed. (e) Treg to CD4+ T-cell ratio at 16 weeks of diet-challenge. Data are presented as the mean±s.e.m.; n=10–12/group. *P<0.05.

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