Figure 4 | International Journal of Oral Science

Figure 4

From: Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate

Figure 4

cAMP regulated the LPLI-mediated proliferation and osteogenic differentiation of hPDL cells. (a) hPDL cells were treated with LPLI at 2 J⋅cm−2 alone or in combination with SQ22536, and then an MTT assay was performed (n=12). (b–e) The effect of cAMP signaling on the expression of osteogenic genes (BMP2, OC, RUNX2 and ALP in sequence) by RT-PCR (n=4). The data are shown as the mean±s.d., and the levels of statistical significance are indicated as follows: *P<0.05, **P<0.01 and ***P<0.001 relative to the LPLI (2 J⋅cm−2)/OIM-2 group. ALP, alkaline phosphatase; hPDL, human periodontal ligament; LPLI, low-power laser irradiation; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; OIM, osteo-induction medium; RT-PCR, reverse transcription-polymerase chain reaction; OD, optical density.

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