Figure 4

Reverse transcription-polymerase chain reaction. Messenger RNA (mRNA) expression of both CD44 and Bmi-1 (white arrows) was observed in the sphere-forming-like cells from the VX2 buccal carcinoma, but CD133 and ALDH1 were not expressed; mRNA expression of Nestin, Oct4 and Rex-1 was also observed (white arrows).