Figure 8
In vivo tumorigenesis assay of the first-passage sphere-forming-like cells. Different numbers of the first-passage sphere-forming-like cells from the VX2 rabbit buccal carcinoma were subcutaneously injected into nude mice. Representative image showing tumour xenograft formation (a) and representative micrograph showing the histological differentiation of the tumour xenograft (b, haematoxylin–eosin stain); 103 cells could produce visible tumour formation within 7 weeks post-transplantation (c).
